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Literature summary for 3.8.1.2 extracted from
- Detection of halogenated organic compounds using immobilized thermophilic dehalogenase (2009), Anal. Bioanal. Chem., 395, 1173-1178.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is covalently linked to an N-hydroxysuccinimidyl Sepharose resin to construct a highly specific sensor with long shelf life for the detection of L-2-haloacids | Sulfurisphaera tokodaii |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme retains approximately 30% of the overall activity after immobilization | Sulfurisphaera tokodaii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
(S)-2-chloropropionic acid | pH 9.0, 50°C, soluble enzyme | Sulfurisphaera tokodaii |  |
| 1.6 | - |
(S)-2-chloropropionic acid | pH 9.0, 22°C, immobilized enzyme | Sulfurisphaera tokodaii | |
| 2.7 | - |
(S)-2-chloropropionic acid | pH 9.0, 37°C, immobilized enzyme | Sulfurisphaera tokodaii | |
| 4.9 | - |
(S)-2-chloropropionic acid | pH 9.0, 50°C, immobilized enzyme | Sulfurisphaera tokodaii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.9 | - |
substrate: (S)-2-chloropropionic acid, pH 9.0, 22°C, immobilized enzyme | Sulfurisphaera tokodaii |
| 3 | - |
substrate: (S)-2-chloropropionic acid, pH 9.0, 37°C, immobilized enzyme | Sulfurisphaera tokodaii |
| 6.3 | - |
substrate: (S)-2-chloropropionic acid, pH 9.0, 50°C, immobilized enzyme | Sulfurisphaera tokodaii |
| 20 | - |
substrate: (S)-2-chloropropionic acid, pH 9.0, 50°C, soluble enzyme | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-2-chloropropionic acid + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-HADST | - |
Sulfurisphaera tokodaii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
soluble and immobilized enzyme | Sulfurisphaera tokodaii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10.5 | pH 8.0: about 50% of maximal activity, pH 10.5: about 80% of maximal activity | Sulfurisphaera tokodaii |
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